Land-use influences phosphatase gene microdiversity in soils.

نویسندگان

  • Andrew L Neal
  • Maike Rossmann
  • Charles Brearley
  • Elsy Akkari
  • Cervin Guyomar
  • Ian M Clark
  • Elisa Allen
  • Penny R Hirsch
چکیده

Phosphorus cycling exerts significant influence upon soil fertility and productivity - processes largely controlled by microbial activity. We adopted phenotypic and metagenomic approaches to investigate phosphatase genes within soils. Microbial communities in bare fallowed soil showed a marked capacity to utilise phytate for growth compared with arable or grassland soil communities. Bare fallowed soil contained lowest concentrations of orthophosphate. Analysis of metagenomes indicated phoA, phoD and phoX, and histidine acid and cysteine phytase genes were most abundant in grassland soil which contained the greatest amount of NaOH-EDTA extractable orthophosphate. Beta-propeller phytase genes were most abundant in bare fallowed soil. Phylogenetic analysis of metagenome sequences indicated the phenotypic shift observed in the capacity to mineralise phytate in bare fallow soil was accompanied by an increase in phoD, phoX and beta-propeller phytase genes coding for exoenzymes. However, there was a remarkable degree of genetic similarity across the soils despite the differences in land-use. Predicted extracellular ecotypes were distributed across a greater range of soil structure than predicted intracellular ecotypes, suggesting that microbial communities subject to the dual stresses of low nutrient availability and reduced access to organic material in bare fallowed soils rely upon the action of exoenzymes.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Relationships Between land Use and Arbuscular Mycorrhizal (AM) Spore Abundance in Calcareous Soils

This study was conducted to determine soil properties that correlate with the arbuscular mycorrhizal fungal (AMF) spore numbers in semiarid calcareous soils of Hamadan province in northwestern of Iran. Soil samples from six sites managed differently were collected from a 0 to 30 cm depth. The results showed that land use and management systems had a significant effect on AMF spore number in soi...

متن کامل

Differences in Arbuscular Mycorrhizal Fungal Community Composition in Soils of Three Land Use Types in Subtropical Hilly Area of Southern China

Land use type is key factor in restoring the degraded soils due to its impact on soil chemical properties and microbial community. In this study, the influences of land use type on arbuscular mycorrhizal fungal (AMF) community and soil chemical properties were assessed in a long-run experimental station in subtropical hilly area of southern China. Soil samples were collected from forest land, o...

متن کامل

Relative impacts of land-use, management intensity and fertilization upon soil microbial community structure in agricultural systems

Soil microbial communities under three agricultural management systems (conventionally tilled cropland, hayed pasture, and grazed pasture) and two fertilizer systems (inorganic fertilizer and poultry litter) were compared to that of a w150-y-old forest near Watkinsville, Georgia. Both 16S rRNA gene clone libraries and phospholipid fatty acid (PLFA) analyses indicated that the structure and comp...

متن کامل

The effect of forest degradation and land use change on some soil biological indexes (case study: Persian oak (Quercus brantii Lindl) forests in Fars province)

To understand the effects of forest degradation and their conversion to farmlands, the current research was conducted with emphasis on some soil biological indexes. In this regard, two different land use including an oak natural stand and a farm next to the forest and under cultivation since 20 years ago were surveyed. Within the each land use, three sampling plots with 10×10 meter dimension we...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Environmental microbiology

دوره 19 7  شماره 

صفحات  -

تاریخ انتشار 2017